Part:BBa_K1940001:Design

microRNA-34c detector 1.0

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 186
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 50
  Illegal BsaI.rc site found at 183
  Illegal BsaI.rc site found at 864
  

Design Notes

We had tested that anti-microRNA-34c in the position was most effective than after RBS or GFP.

Source

composited